Plasmid

Part:BBa_K890000:Design

Designed by: James Bevington   Group: iGEM12_UGA1   (2012-10-03)


methanococcus shuttle vector pAW42


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 234
    Illegal XbaI site found at 243
    Illegal SpeI site found at 4948
    Illegal PstI site found at 772
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 4948
    Illegal PstI site found at 772
    Illegal NotI site found at 959
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 221
    Illegal BamHI site found at 753
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 234
    Illegal XbaI site found at 243
    Illegal SpeI site found at 4948
    Illegal PstI site found at 772
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 234
    Illegal XbaI site found at 243
    Illegal SpeI site found at 4948
    Illegal PstI site found at 772
    Illegal NgoMIV site found at 203
    Illegal AgeI site found at 795
    Illegal AgeI site found at 971
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3129
    Illegal BsaI.rc site found at 1512
    Illegal BsaI.rc site found at 1841
    Illegal SapI site found at 4793


Design Notes

Clone DNA downstream from a strong promoter, Nde1 site.


Source

Walters, Alison D., Sarah Smith, and James Chong. "Shuttle Vector System for Methanococcus 

   maripaludis with Improved Transformation Efficiency." Applied and Environmental Microbiology 

   77.7 (2011): 2549-51. Print.


References